|Goodwin, Stephen - Steve|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/1999
Publication Date: N/A
Technical Abstract: Differential display was used to compare gene expression in resistant and susceptible wheat lines following inoculation with the septoria pathogen, Mycosphaerella graminicola. Treatments were Yecora Rojo (susceptible) and Tadinia (resistant) uninoculated, and the same two cultivars inoculated with M. graminicola. Tadinia possesses the Stb4 gene for resistance. Leaf ftissue was collected at 0, 3, 6, 12, 24, 48 and 96 hours after inoculation with a final sampling after 4 weeks. Total RNA was extracted and subjected to differential display using four specific and eight random primers. Following electrophoresis on denaturing sequencing gels and autoradiography, differentially expressed genes were excised, cloned and sequenced. Most sequences had no matches in GenBank. However, three clones had high similarity to a pathogenesis-related protein from Phaseolus vulgaris. Most genes were expressed in both the susceptible and the resistant host. Experiments are underway to confirm the timing of differential expression using both traditional Northern and reverse-Northern analyses.