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ARS Home » Research » Publications at this Location » Publication #100995
Title: STUDIES ON A NEW CARLA VIRUS FROM POTATO

Author
item Goth, Robert
item ELLIS, P. - PHYTO DIAGNOSTICS CO.
item DE VILLIERS, G. - PHYTO DIAGNOSTICS CO.
item Goins, Ernest
item WRIGHT, N. - PHYTO DIAGNOSTICS CO.
item Ballesteros, Maria

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In 1992, the Scottish Agricultural Science Agency detected a carla virus (code name RLSV) also called Potato Latent Carlavirus (PLCV) with distinct serological and biological characteristics in the cv. Red LaSoda. In 1993, the long filamentous rod-shaped virus particle was observed in a clone of the potato cv. Red LaSoda during routine electron microscope testing of accessions in the Vancouver Collection of Virus-Free Potatoes being grown in the California winter tests. Although the particle size of RLSV/PLCV is similar to Potato Virus S and Potato Virus M, it is serologically distinct. A highly specific monoclonal antibody, Mab 4E12, was developed for RLSV/PLCV. In 1997 and 1998, 137 potato accessions in the US National Varietal Collection maintained at Presque Isle, ME, were assayed for RLSV with TAS ELISA using the 4E12 Mab. Three cvs., High Plains, Platte, and Red LaSoda, tested positive. Additional systemic hosts for RLSV/PLCV are Nicotiana benthimiana, N. megalosiphon, and N. occidentalis. TAS ELISA using the 4E12 Mab is the standardized test for RLSV/PLCV in Canada.