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Title: DIFFERENTIATING FOOT-AND-MOUTH DISEASE VIRUS-INFECTED FROM VACCINATED ANIMALS WITH BACULOVIRUS-EXPRESSED SPECIFIC PROTEINS

Author
item MEZENCIO, J-M - FORMER PIADC EMPLOYEE
item BABCOCK, GWEN - USDA-APHIS, FADDL, PIADC
item MEYER, RICHARD - USDA-APHIS, FADDL, PIADC
item LUBROTH, JUAN - USDA-APHIS, FADDL, PIADC
item SALT, J - PIRBRIGHT LAB, ENGLAND
item NEWMAN, JOHN-F - FORMER PIADC EMPLOYEE
item Brown, Fred

Submitted to: The Veterinary Quarterly
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/17/1997
Publication Date: N/A
Citation: N/A

Interpretive Summary: This paper was presented at a meeting of the European Community to discuss the results of a joint program to distinguish between animals infected with FMDV and those vaccinated against the disease. In preliminary studies with a small number of cattle and swine we had shown that antibodies against 2C, one of the non-structural proteins of the virus could be detected in animals convalescent from FMD, but not in vaccinated animals. In an extension of this work to include several hundred animals, we have confirmed the validity of the test. Moreover, the protein can be produced in a baculovirus vector so it could be made available from a laboratory not restricted by FMD security regulations.

Technical Abstract: We had shown in preliminary studies with a small number of animals that antibodies against 2C could be detected in cattle and pigs which had been infected with FMDV but not in animals which had been vaccinated against the disease. To determine whether this test was generally applicable, sera from several hundred animals which had been vaccinated with different products in many countries have been tested in an ELISA using baculovirus expressed 2C. Our results show that only 1-2% of the sera gave a positive reaction by this method. In contrast, 100% of sera from convalescent animals gave a positive reaction.