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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #326701
Title: Identification and characterisation of Dof transcription factors in the cucumber genome

Author
item WEN, CHANG-LONG - Beijing Academy Of Agricultural Sciences
item CHENG, QING - Beijing Academy Of Agricultural Sciences
item ZHAO, LIQUN - China Agricultural University
item MAO, AIJUN - Beijing Academy Of Agricultural Sciences
item YANG, JINGJING - Beijing Academy Of Agricultural Sciences
item YU, SHUANCANG - Beijing Academy Of Agricultural Sciences
item Weng, Yiqun
item XU, YONG - Beijing Academy Of Agricultural Sciences

Submitted to: Scientific Reports
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/25/2016
Publication Date: 3/16/2016
Publication URL: http://handle.nal.usda.gov/10113/62240
Citation: Wen, C., Cheng, Q., Zhao, L., Mao, A., Yang, J., Yu, S., Weng, Y., Xu, Y. 2016. Identification and characterisation of Dof transcription factors in the cucumber genome. Scientific Reports. 6:23072. doi: 10.1038/srep23072.

Interpretive Summary: Although candidate genes for resistances to several pathogens in cucumber have been reported, the underlying defence mechanisms remain unclear. The Dof (DNA-binding with one finger) proteins are a group of plant-specific transcription factors that have been shown to play significant roles in plant growth, development, and responses to biotic and abiotic stresses. Dof genes can promote large-scale expression of defence-related genes at whole genome level. The gene families have been identified and characterized in several plant species, but not in cucumber. In the present study, we identified 36 CsDof gene members from the cucumber draft genome which could be classified into eight groups. The proportions of the CsDof family genes, duplication events, chromosomal locations, cis-elements and miRNA target sites were comprehensively investigated. Consequently, we analysed the expression patterns of CsDof genes in specific tissues and their response to two biotic stresses (watermelon mosaic virus and downy mildew). These results indicated that CsDof may be involved in resistance to biotic stresses in cucumber.

Technical Abstract: Cucumber is susceptible to many foliage diseases. Although candidate genes for resistances to several pathogens in cucumber have been reported, the underlying defence mechanisms remain unclear. The Dof (DNA-binding with one finger) proteins are a group of plant-specific transcription factors that have been shown to play significant roles in plant growth, development, and responses to biotic and abiotic stresses. Dof genes can promote large-scale expression of defence-related genes at whole genome level. The gene families have been identified and characterized in several plant species, but not in cucumber. In the present study, we identified 36 CsDof gene members from the cucumber draft genome which could be classified into eight groups. The proportions of the CsDof family genes, duplication events, chromosomal locations, cis-elements and miRNA target sites were comprehensively investigated. Consequently, we analysed the expression patterns of CsDof genes in specific tissues and their response to two biotic stresses (watermelon mosaic virus and downy mildew). These results indicated that CsDof may be involved in resistance to biotic stresses in cucumber.