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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research Unit » Research » Publications at this Location » Publication #322123

Title: pltM is important in the phloroglucinol-mediated crosstalk between biosynthetic gene clusters for the antibiotics 2,4-diacetylphloroglucinol and pyoluteorin in Pseudomonas protegens Pf-5

Author
item YAN, QING - Oregon State University
item CHANG, JEFF - Oregon State University
item Loper, Joyce
item Clifford, Jennifer

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2015
Publication Date: 6/30/2015
Citation: Yan, Q., Chang, J.H., Loper, J.E., Clifford, J.M. 2015. pltM is important in the phloroglucinol-mediated crosstalk between biosynthetic gene clusters for the antibiotics 2,4-diacetylphloroglucinol and pyoluteorin in Pseudomonas protegens Pf-5 A functional gene cluster for toxoflavin biosynthesis in the genome of the soil bacterium Pseudomonas protegens Pf-5 [abstract]. Phytobiomes 2015: Designing a New Paradigm for Crop Improvement.

Interpretive Summary:

Technical Abstract: Pseudomonas protegens strain Pf-5 is a well-characterized rhizosphere bacterium known for its production of a diverse spectrum of antimicrobial secondary metabolites. The production of two of these metabolites, 2,4-diacetylphloroglucinol (2,4-DAPG) and pyoluteorin, is coordinately regulated. Each of the two metabolites functions as an intercellular signal, inducing the expression of genes responsible for its own biosynthesis. Our previous study indicate that phloroglucinol, an intermediate in the synthesis of 2,4-DAPG, plays an important role in the regulation of the transcription of the pyoluteorin biosynthetic genes. But the mechanism of phloroglucinol in the regulation of pyoluteorin biosynthesis is still unclear. Here we report that phloroglucinol-mediated positive regulation of the pyoluteorin biosynthesis gene pltA by the linked transcriptional regulator PltR was abolished by a mutation in pltM, which encodes a putative halogenase. A pltM mutant of Pf-5 did not produce pyoluteorin or express pltA, even in the presence of phloroglucinol. Purified PltM converted phloroglucinol into a new compound in vitro. This compound remarkably induced pltA expression in a pltM deficient strain. We are currently working on the chemical structure of the compound by HPLC-MS.