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ARS Home » Southeast Area » Stoneville, Mississippi » Crop Genetics Research » Research » Publications at this Location » Publication #302793
Title: Mapping of QTL associated with seed amino acids content in MD96-5722 by "Spencer" RIL population of soybean using SNP markers

Author
item LAILA, KHANDAKER - Fayetteville State University
item AKOND, MASUM - Southern Illinois University
item LIU, SHIMING - Southern Illinois University
item KANTARTZI, STELLA - Southern Illinois University
item MEKSEM, KHALID - Southern Illinois University
item Bellaloui, Nacer
item LIGHTFOOT, DAVID - Southern Illinois University
item KASSEM, ABDELMAJID - Fayetteville State University

Submitted to: Food and Nutrition Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/8/2015
Publication Date: 8/13/2015
Citation: Laila, K., Akond, M., Liu, S., Kantartzi, S.K., Meksem, K., Bellaloui, N., Lightfoot, D.A., Kassem, A.M. 2015. Mapping of QTL associated with seed amino acids content in MD96-5722 by "Spencer" RIL population of soybean using SNP markers. Food and Nutrition Sciences. 974-984.

Interpretive Summary: Although soybean seeds contain essential amino acids required for human and livestock nutrition, increasing the levels and improving the quality of soybean amino acids are still a challenge. Therefore, identifying the genes controlling amino acids in seeds is essential. The objective of this research was to identify regions of the genes controlling amino acids in soybean breeding populations using DNA markers. The results showed that thirteen chromosomal regions were identified for seed amino acids. This information facilitates soybean breeding to select for high level and quality of amino acids to help meet industry needs.

Technical Abstract: Soybean seeds are major sources of essential amino acids, protein, and fatty acids. Limited information is available on the genetic analysis of amino acid composition in soybean. Therefore, the objective of this study was to identify genomic regions containing quantitative trait loci (QTL) controlling different amino acid content in soybean seeds. To achieve this objective, 92 F5:7 recombinant inbred lines (RIL) developed from a cross of MD 96-5722 and ‘Spencer’ were used and genotyped using SNP markers developed from the Illumina Infinium SoySNP6K BeadChip array. A total of 13 QTL were identified with three QTL for threonine on the linkage group (LG) A1/ chromosome (Chr) 5, LG C2/Chr6, and LG B2/Chr14. Two QTL were identified for each of the amino acids proline (LG D1a/Chr1 and LG B2/Chr14), serine (LG A1/Chr5 and LG C2/Chr6), tryptophan (LG K/Chr9 and LG G/Chr18), and cysteine (LG A1/Chr5 and LG K/Chr9). One QTL was identified for arginine (LG N/Chr3) and histidine (LG J/Chr16) content. This information is significant in that the new QTL for amino acids in soybean could facilitate developing soybean cultivars with desired amino acid profiles to help meet industry needs.