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ARS Home » Plains Area » Clay Center, Nebraska » U.S. Meat Animal Research Center » Livestock Bio-Systems » Research » Publications at this Location » Publication #291232
Title: Laser capture microdissection coupled with RNA-seq reveals that short cuboidal trophoblast cells of the porcine placenta possess a transcriptome consistent with a migratory cell type

Author
item McNeel, Anthony
item Chen, Celine
item Schroeder, Steven - Steve
item Sonstegard, Tad
item Dawson, Harry
item Vallet, Jeff

Submitted to: Society for the Study of Reproduction Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/28/2013
Publication Date: 7/1/2013
Citation: McNeel, A.K., Chen, C.T., Schroeder, S.G., Sonstegard, T.S., Dawson, H.D., Vallet, J.L. 2013. Laser capture microdissection coupled with RNA-seq reveals that short cuboidal trophoblast cells of the porcine placenta possess a transcriptome consistent with a migratory cell type [abstract]. Biology of Reproduction Supplement (46th Annual Meeting of the Society for the Study of Reproduction). pp. 239-240 (Abstract #516).

Interpretive Summary:

Technical Abstract: The porcine placenta is classified as epitheliochorial with a diffuse distribution of chorionic villi that do not invade maternal tissues. Thus, the maternal and fetal bloodstreams are separated by six distinct tissue layers. The porcine fetal-maternal interface (FMI) is substantially folded to increase the interactive surface area, becomes more folded as gestation advances, and it is likely that the extent of folding plays a significant role in fetal survival. We have reported that the depth folding along the FMI is inversely related to the size of the fetus. We have also shown that the abundance of enzymes known to degrade the fetal extracellular matrix of the placenta increases with advancing gestation and in placentas of small fetuses. Furthermore, we have evidence demonstrating there is differential localization of heparanase (HSPE) gene expression within the fetal maternal interface, which corresponds to phenotypic differences in trophoblast epithelial cells (short cuboidal: SC; tall columnar: TC). We hypothesized that the phenotypically and spatially distinct trophoblast epithelial cells differ in their contribution to fold development, differing in their capacity for cell migration and that these differences would be reflected in their transcriptome. Populations of phenotypically distinct SC and TC trophoblast epithelial cells were isolated from day 85 ± 3 cross-sections of porcine placentas (n = 4) using laser capture microdissection. RNA-seq was performed on an Illumina Hi-seq 2000 and reads were mapped to the Sus Scrofa genome (v 9.2). Expression was conveyed using the RPKM method and 7398 unique genes/sequences met the minimum expression threshold (1.8 RPKM). Expression data was analyzed by one-way ANOVA with adaptive false discovery rate via JMP Genomics (v 5.0) and differences were considered statistically significant when P