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ARS Home » Southeast Area » Fayetteville, Arkansas » Poultry Production and Product Safety Research » Research » Publications at this Location » Publication #276274
Title: Reduction of Salmonella Enterica serovar Enteritidis colonization in 20-day-old broiler chickens by the plant derived compounds trans-cinnamaldehyde and eugenol

Author
item KOLLANOOR, JOHNY - University Of Connecticut
item BASKARAN, S - University Of Connecticut
item AMALARADJOU, M - University Of Connecticut
item BAPOOR, S - University Of Connecticut
item MARCH, B - University Of Connecticut
item VALIPE, S - University Of Connecticut
item HOAGLAND, T - University Of Connecticut
item DARRE, M - University Of Connecticut
item SCHREIBER, D - University Of Connecticut
item KHAN, M - University Of Connecticut
item Donoghue, Ann - Annie
item DONOGHUE, D - University Of Arkansas
item VENKITANARAYANAN, K - University Of Connecticut

Submitted to: Applied and Environmental Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/2/2012
Publication Date: 2/10/2012
Citation: Kollanoor, J.A., Baskaran, S.A., Amalaradjou, M.A., Bapoor, S., March, B., Valipe, S.R., Hoagland, T.A., Darre, M.J., Schreiber, D.T., Khan, M.A., Donoghue, A.M., Donoghue, D.J., Venkitanarayanan, K. 2012. Reduction of Salmonella Enterica serovar Enteritidis colonization in 20-day-old broiler chickens by the plant derived compounds trans-cinnamaldehyde and eugenol. Applied and Environmental Microbiology. 78(8):2981-2987.

Interpretive Summary: This study investigated the efficacy of trans-cinnamaldehyde (TC) and eugenol (EG) for reducing Salmonella Enteritidis (SE) colonization in broiler chicks. Feed intake and body weight were not significantly different for TC treatments. However, EG-treated birds had significantly lower body weights compared to the control. Follow up in vitro experiments revealed that the sub-inhibitory concentrations (SIC – concentrations that do not inhibit Salmonella growth) of TC and EG reduced motility and invasive abilities of SE, and down-regulated expression of motility genes flhC and motA, and invasion genes hilA, hilD and invF. The results suggest that supplementation of TC and EG through feed can effectively reduce SE colonization in chickens.

Technical Abstract: This study investigated the efficacy of trans-cinnamaldehyde (TC) and eugenol (EG) for reducing Salmonella Enteritidis (SE) colonization in broiler chicks. In three separate experiments for each compound, day-old, chicks (N=75/experiment) were randomly assigned to five treatments (n=15/treatment): a negative control (-SE, -TC or EG), a compound control (-SE, +TC or EG at 0.75% or 1%, respectively), a positive control (+SE, -TC or EG), a low dose treatment (+SE, +0.5% TC or 0.75% EG) and a high dose treatment (+SE, +0.75% TC or 1% EG). On day 0, birds were tested for any inherent Salmonella (n=5/experiment). On day 8, birds were inoculated with 8.0 log10 CFU SE by crop gavage, and cecal colonization by SE was ascertained (n=10/experiment) after 24 h. Six birds from each treatment were euthanized on days 7 and 10 after inoculation, and SE populations in the cecum were determined. TC at 0.5 or 0.75%, and EG at 0.75 or 1% consistently reduced SE populations in cecum after 10 days of infection, in all experiments. Feed intake and body weight were not significantly different for TC treatments. However, EG treated birds had significantly lower body weights compared to the control. Follow up in vitro experiments revealed that the sub-inhibitory concentrations (SIC – concentrations that do not inhibit Salmonella growth) of TC and EG reduced motility and invasive abilities of SE, and down-regulated expression of motility genes flhC and motA, and invasion genes hilA, hilD and invF. The results suggest that supplementation of TC and EG through feed can effectively reduce SE colonization in chickens.