Location: Sunflower and Plant Biology Research
Project Number: 3060-21220-031-012-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2016
End Date: Dec 31, 2020
Objective:
1) Identify and/or verify resistance to white mold in wide cross and adapted dry and snap bean lines; 2) Assess the phenotypic and genotypic variation, including fungicide sensitivity in bean isolates of Sclerotinia sclerotiorum collected from major production areas.
Approach:
Multiple field locations and greenhouse straw tests will be used to screen for white mold reaction of putative sources of resistance. These lines will be provided by bean breeders and plant pathologists from recombinant inbred line populations, interspecific hybridizations, and advanced breeding lines. Field plots will be located in areas of Oregon, Michigan, Wisconsin, France, North Dakota, Nebraska, and Washington with a history of white mold. Five field locations plus Colorado will conduct greenhouse straw tests. The population genetics and fungicide sensitivity of 366 S. sclerotiorum greenhouse and field isolates collected over years at screening trial sites and in bean grower fields will be determined by haplotypes identified by the use of new microsatellite-specific primers. The aggressiveness of the new collection of S. sclerotiorum isolates will be determined by the straw test. Fungicide sensitivity using mycelium and ascospores will be determined for all isolates to guide the use of this disease management tool. Pathogen population knowledge will guide use of relevant specific screening isolates for breeders and plant pathologists.
