Location: Diet, Genomics and Immunology Lab
Project Number: 8040-51530-056-07
Start Date: Aug 01, 2013
End Date: Nov 01, 2014
Various sources of cocoa products normally ingested by American consumers will be evaluated for the quantity and diversity of flavonoids. A representative source will be added to an obesogenic diet fed to pigs at levels comparable to human daily intake and flavonoid absorption and derivative molecules will be followed in the urine and serum by metabolomic measurements. The pre-biotic potential of cocoa will be associated with inherent changes in intestinal microbiota, and in combination with probiotic Lactobacillus and Bifidobacterium strains fed to pigs. Changes in inflammatory markers will be measured systemically in the blood and locally by gene expression; immune function and intestinal physiology will be measured using cellular and antibody assays and tissue resistance, respectively.