Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Research Project: Profiling Neonicotinoid Residues from Seed Treatments and Foliar Applications When Applied to Corn, Cotton, and Soybean Grown in the Southern Usa

Location: Southern Horticultural Research

Project Number: 6404-21430-002-13
Project Type: Specific Cooperative Agreement

Start Date: Sep 09, 2013
End Date: Sep 08, 2018

Objective:
The objective of this cooperative research is to profile certain insecticidal residues from seed treatments and foliar applications when applied to corn, cotton, and soybeans to ultimately develop better management decisions to minimize exposure to honey bees.

Approach:
All soil and plant tissue samples will be collected from corn, cotton, and soybean trials located at the Delta Research and Extension Center at Stoneville, MS. Treatments will be clothiandin seed treatments at recommended rates and a non-treated control. Plant tissue samples will be collected from corn plots at the V1, V3-V4, V5, V7, V10, and R1 growth stages. Additionally, soil samples will be collected at the V1 and R1 growth stages. The study will be planted in a randomized complete block(RCB) design with 4 replications. Leaf samples will be collected from the uppermost fully expanded leaf. The treatments will be the commercial rates of imidacloprid + thiodicarb (Aeris) and thiamethoxam + abamectin (Avicta) seed treatments and a non-treated control. Leaf samples will be collected at True Leaf (TL) until bloom occurs. The first full expanded leaf will be taken from a minimum of 10 leaves per plot. Additionally, soil samples will be collected at the 1st true leaf stage and at first bloom. The study will be planted in a RCB design with 4 replications. Leaf samples will be collected from the uppermost fully expanded leaf. This will depend on plant stage but should be no less than 20 plants at smaller stages and no fewer than 10 plants at older stages. Other treatments included in this study will be commercial rates of thiamethoxam (Cruiser Maxx Soybean) and clothiandin (Poncho/NipSit) seed treatments and a non-treated control. Plant tissue samples will be collected from corn plots at both the vegetative and reproductive growth stages. Additionally, soil samples will be collected at the V1 and R1 growth stages. The study will be planted in a RCB design with 4 replications. Leaf samples will be collected from the uppermost fully expanded trifoliate, with the exception of the VC growth stage. Plots of soybeans planted without an insecticide seed treatment will be utilized for this study. Experimental design will be a RCB with 4 replications. The treatments will include a non-treated control and a tank mix of beta-cyfluthrin + imidacloprid and lambda cyhalothrin + thiamethoxam. The two products can be applied to the same plot because analysis tests for both compounds and can quantify the concentration of each from the same sample. Applications will be made at R1 and soybean flower samples (100/plot) will be collected 7-10 days after application. Plots of cotton planted without an insecticide seed treatment will be utilized for this study. Experimental design will be a RCB with 4 replications. The treatments will include a non-treated control and a tank mix of imidacloprid, thiamethoxam, and sulfoxaflor. These products can be applied to the same plot because analysis tests for all three compounds and can quantify the concentration of each from the same sample. Managed hives will be placed in proximity to these crops. Samples of bees, pollen loads, and stored pollen will be collected and analyzed for neonicotinoid residues. In addition, colony health will be monitored throughout the growing season using methods described at www.colonymonitoring.com. All samples will be analyzed at the USDA, AMS, National Science Laboratory, Gastonia, NC.

Last Modified: 4/17/2014
Footer Content Back to Top of Page