Location: Food and Feed Safety Research
Project Number: 6054-42000-026-03
Start Date: Jan 15, 2014
End Date: Jun 30, 2018
The atoxigenic strains of A. flavus currently in development in Nigeria will be the focus of these studies. Techniques useful in detecting and quantifying use of the target atoxigenic strains on the maize crop will be developed to provide performance data based on crop sampling, isolation of deoxyribonucleic acid (DNA) and quantitative pyrosequencing. The work will include validation and refinement of assays, as well as, assistance in the training of collaborating Africa based scientists and the building of capacity in the target nation. Field and laboratory experience will be used to troubleshoot the assays and optimize them for the intended use in assessment of biological control activities.