Project Number: 5030-21000-059-00
Start Date: Feb 05, 2013
End Date: Feb 04, 2018
Extensive collaboration efforts on the part of 52 current cooperators from the private, public and international sectors are required to broaden the germplasm base in effective ways that provide germplasm of use for food, feed, fuel, and industrial applications by producers and end-users. The Coordinator serves as the liaison for collaborators and the Technical Steering Group (TSG), selects germplasm, facilitates germplasm acquisition and stakeholder interactions, arranges for in-kind-support, information sharing, and technology transfer. The Ames location will develop germplasm derived from 50% and 25% exotic breeding crosses developed by crossing tropical and temperate racial accessions with adapted, elite proprietary Corn Belt lines. Breeding crosses will also be developed from GEM x GEM releases, and other models to maximize useful exotic germplasm contributions. Approximately 100 breeding crosses will be evaluated in three environments and ~30 selected annually for development in Ames or collaborator nurseries. Approximately 2,000 S2 top crosses will be made and evaluated annually in yield trials, disease nurseries, and for value-added traits such as ethanol, protein, oil, and starch. Germplasm will be further evaluated by a network of cooperators with expertise, facilities, and favorable selection environments for the traits of interest. Important traits include mycotoxin resistance, abiotic stress tolerance, and insect resistance. A concerted effort will be made to evaluate and develop germplasm for globally important diseases through our international network of cooperators. Germplasm lines will be released to cooperators and selected lines registered and publicly released. Released lines will be maintained by the National Plant Germplasm System. A tropical accession, CUBA164, will be studied for adaptability to high plant densities using a mapping population of ~400 BC1S2 plants that were top crossed to a common tester. Tissue will be sampled from the BC1S2 plants and genotyped by a collaborator. The genomic information from this study will be used to develop a genomic model to select lines for recycling. An effort will be made to develop lines derived from approximately 300 races of maize to broadly represent the allelic diversity of the maize races. Initial crosses of racial accessions with expired PVP lines or other public lines and one backcross to the adapted line (BC1) are made in winter nurseries. The resulting BC1 generation will be used for selecting lines in Midwest nurseries in order to release a unique set of (F5 generation) adapted, racial derivative lines for research and discovery. BC1 populations will also be used to develop doubled haploids (DH), and will be further studied by phenotypic evaluation and genotypic characterization using current technologies such as genotype by sequencing (GBS). Genotypes will be screened in selected environments to maximize selection for priority agronomic, biotic and abiotic stress, reduced mycotoxin, and value-added traits.