Location: Crops Pathology and Genetics Research
Project Number: 2032-21000-021-00
Start Date: Apr 17, 2013
End Date: Apr 16, 2018
Goal 1A: The mutation density of ~4 mutants per Mb using seed mutagenesis will be improved through modification of current protocols. Goal 1B: Two approaches will be taken: 1) mutagenesis of tetraploid rice; and 2) seed mutagenesis at mutagen doses that elicit high or complete pollen sterility followed by crossing with wild-type pollen. Goal 1C: The GECN in rice will vary depending on the mutagen, the dose, and the inflorescence (primary vs. secondary tillers). Goal 2A: Phenotypic data from a systematic evaluation of M2 and M3 mutants will add value to the mutant resource and provide users with additional criteria for selecting mutant lines for further characterization and/or incorporation into variety development programs. Goal 2B: Generate in silico mutation profiles of 1,500 to 2,000 independent M2 rice mutants by exome sequencing existing and newly created mutant populations. Goal 2C: Many valuable mutations/mutant phenotypes may be missed in early generations (i.e. M2 or M3). Fixed lines (=M6 generation) will provide researchers a long-term resource for evaluation of traits under field conditions, that require multiple replications (e.g. locations and years), or that are destructive in nature. Goal 3A: Mutations that improve grain quality (milling, cooking, eating, and nutritional) or agronomic performance of rice or that result in novel phenotypes of commercial value may be identified through phenotypic evaluation (i.e. forward genetics). Goal 3B: Through a combination of TILLING and targeted exome sequencing, mutations in genes of interest will be identified and the corresponding mutants will be characterized. Goal 3C: Develop a functional genomics/variety development pipeline by applying next-gen sequencing-based methods in combination with accelerated mapping population development to identify mutant phenotypes, map and clone the underlying genes, and generate enhanced germplasm for breeding programs.