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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development of a Taqman Array Card for Multiplex Detection of Citrus Pathogens by Real-Time Pcr

Location: Crop Diseases, Pests and Genetics

Project Number: 5302-22000-011-06
Project Type: Trust

Start Date: Jan 01, 2013
End Date: Dec 31, 2014

Objective:
Develop a custom multiplex TaqMan® Array card as a standardized real-time polymerase chain reaction assay for simultaneous detection of RNA and DNA citrus pathogens of regulatory importance to the California citrus nurseries.

Approach:
Use and modify commercial kits which capture nucleic acid on magnetic beads during robotic extraction to yield high quality total nucleic acid; utilize existing literature and validate or improve real-time PCR detection of specific RNA and DNA targets; develop a custom micro-fluidic card with multiple pathogen target signatures (primer sets) for amplicon reaction with TaqMan® probes; use a block specifically designed to conduct qPCR with 384-well microfluidic cards.

Last Modified: 4/23/2014
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