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United States Department of Agriculture

Agricultural Research Service

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Research Project: A Phage/prophage-Based Pcr System for Sensitive and Specific Detection of “candidatus Liberibacter Asiaticus and Spiroplasma

Location: Crop Diseases, Pests and Genetics

Project Number: 2034-22000-010-20
Project Type: Trust

Start Date: Oct 01, 2012
End Date: Sep 30, 2016

The proposed research project is to develop PCR detection systems that are highly sensitive and specific in detection of “Ca. L. asiaticus” and S. citri.

1. Identification and characterization of phages/prophages in “Ca. L. asiaticus” and S. citri through data-mining from available DNA sequences. 2. Selection and evaluation of candidate phage/prophage genes for their capacity in pathogen detection. 3. Testing the phage/prophage-based detection systems using clinical field insect and plant DNA samples.

Last Modified: 11/28/2015
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