Project Number: 3012-21000-014-05
Start Date: Oct 01, 2011
End Date: Sep 30, 2015
This research project will develop and implement technologies to cryopreserve critical clones included in the Citrus Clonal Protection Program in Riverside, California. Preliminary data suggests that diverse Citrus clones can be placed into long-term liquid nitrogen storage and then recovered on-demand using modified shoot tip micrografting techniques. Cryopreserved shoot tips can remain viable for dozens of years. This research project will establish a long-term cryoexposure experiment to confirm viability after extended lengths of LN exposure. The project will also identify optimal cryoprotectant exposure times and source plant materials for improved cryopreservation success. These experiments are critical for the implementation of Citrus cryopreservation methods as a collection conservation strategy. It is critical that materials be backed-up for security purposes before California Citrus genetic resources are threatened by Huanglongbing and other diseases or natural threats.