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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development and Implementation of New Control Strategies for Citrus Huanglongbing (Greening)-Phase II

Location: Subtropical Plant Pathology Research

Project Number: 6034-22000-039-20
Project Type: Reimbursable

Start Date: Dec 06, 2011
End Date: Jul 31, 2014

1. Continue screening and eliminating Huanglongbing (HLB) bacteria from nursery stocks using highly sensitive detection technology, coupled with effective Las-eradicant compounds and thermotherapy. 2. Continue determining if ornamental Murraya species are HLB inoculum reservoirs, and if thermotherapy can be applied to the Murraya industry. 3. Improve culturing of Las bacterium in vitro and conduct functional genomics to elucidate molecular mechanisms of HLB disease. 4. Continue to implement chemical control of citrus HLB by developing cost-effective chemical compounds and application technology.

1. Using the newly-developed super sensitive realtime polymerase chain reaction (PCR) to detect Las bacteria in the germplasms of citrus, and use the two effective chemicals coupled with thermal therapy to treat the budwoods and seeds to obtain real Las-free gerplasms. 2. Using realtime PCR and convential PCR to determine the type(s) of Las bacteria that can survive in Murraya plants and the dynamics of the bacteria in the plants, as well as their possibility to be transmitted to citrus by the asian citrus psyllids. 3. Optimizing the growth factors and conditions for improving the growth rate of Las bacterium in vitro, and knocking out and complementing some of the putative effector genes of Las. 4. Using the chemicals and thermotherapy device, we will apply and modify the application system for practical and cost effective control of citrus HLB.

Last Modified: 3/1/2015
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