Location: Forage and Range Research
Project Number: 5428-21000-014-23
Start Date: Sep 15, 2011
End Date: Sep 14, 2016
This project will focus on response of native and non-native (introduced) plant species when challenged by several abiotic stresses (drought, salt, temperature, and heavy metals). This research will have field, laboratory, and greenhouse components and will involve the mineral analysis of plant species to define their responses to abiotic stress challenge. The response of plant populations and experimental lines (the result of previous breeding from ongoing plant improvement programs) that have been identified in preliminary experiments to possess tolerance and susceptibility to abiotic stresses will be subjected to stresses in replicated trials and will be prepared for analysis by mass spectrometry. For fine-leaf fescue analysis, clonal plants which are + and - endophytes will be evaluated for their response under controlled field (rainout shelter) and greenhouse (overhead delivery system) for water and salt stress challenge, respectively. For orchardgrass and ryegrass experimentals challenged with water (line source) and temperature (cold) stress, whole and biochemical analysis coupled with near-infrared spectroscopy of leaves will be accomplished by wet chemistry to determine water soluble carbohydrate concentration. For plants subjected to heavy metal challenge either in the field or greenhouse, mass spectrometry will be employed to determine the effect of animal manure, sulfur, and denwoody cap treatments on different forms of heavy metals (e.g., Se in Se overburdened soils).