Project Number: 5366-21000-031-04
Start Date: Aug 08, 2011
End Date: Jul 01, 2016
Use current alleles via MAS: We will leverage genotyping services provided by the Triticeae-CAP (T-CAP) project at the USDA Regional Lab, Pullman. In collaboration with Deven See at this facility, we have developed Sequenom assays to characterize stripe rust resistance haplotypes in the following germplasm: (i) doubled haploid (DH) lines derived from crosses of European winter 2 –rows and the BISON, (ii) F4 head rows derived from crosses of BISON resistance with hull-less food types, and (iii) BC1 progeny derived from crosses with Nebraska cold tolerant germplasm. The resistance haplotypes will be validated using field resistance data and used for marker assisted selection (MAS). Discover new resistance QTLs/genes: We will leverage T-CAP Association Mapping (AM) for BSR resistance in the national collection. This germplasm resource will be phenotyped and genotyped in 2012 via T-CAP. Based on the AM, with support from this SCA and in collaboration with personnel at Aberdeen, in the Fall 2012 we will initiate crossing to introgress new QTL alleles/genes into adapted winter germplasm. Confirm, validate, and identify resistant germplasm in addition to OSU program: Because barley stripe rust is not endemic at Aberdeen, Idaho, we will expand our screening of USDA-ARS germplasm for resistance at Corvallis. This will be accomplished by fall-planting in 2011 to ensure optimum epidemic development in spring, 2012. Dissemination and publication: All germplasm will be publicly available: We will prepare and/or co-author peer reviewed publications with USDA-ARS scientists at Aberdeen, Idaho.