SIRNA-MEDIATED KNOCKDOWN/SILENCE POST-TRANSCRIPTIONAL GENE EXPRESSION OF CHICKEN IFN-GAMMA
Animal Biosciences and Biotechnology Laboratory
Project Number: 8042-32000-097-08
Start Date: Jan 01, 2010
End Date: Dec 31, 2014
To explore the possibility of initial stage of host gene manipulation by developing RNA interference technology using primary lymphocytes. We will use RNAi technology to modulate IFN-y response since IFN-y is a key immunoregulatory cytokine of macrophages for mucosal immune response.
Peripheral blood monocytes will be cultured in vitro and treated with siRNA using recently developed RNA interference (RNAi) technology to knockdown/silence post-transcriptional gene expression of chicken IFN-y.