Project Number: 6050-21000-013-00
Start Date: Oct 01, 2008
End Date: Sep 30, 2013
Traits that are known to confer resistance to mites (autogrooming against tracheal mites; VSH or its behavioral subtasks against varroa) will be subjected to microarray analysis to identify genes associated with specific phenotypes. Genes will be further screened for up- and down-regulation using rtPCR assays. New traits of resistance to varroa (reduced invasion by mites into brood cells; brood-mediated suppression of mite reproduction) or to CCD-related agents will be sought by measuring variation among diverse bee sources. Traditional breeding will be used to create honey bees suitable for commercial crop pollination by combining lines having high VSH with commercial stock. Molecular-marker-assisted selection will focus on genetic markers developed earlier for autogrooming and VSH. Simplified methods for queen breeders to select for VSH will be evaluated by correlating VSH expression with changes in brood nest characters during short-term exposure of infested combs. Sustainability of varroa resistance in bees used for migratory crop pollination will be determined by measuring survivability and performance of VSH colonies in cooperation with commercial beekeepers. Recommendations for resistance-based Integrated Pest Management (IPM) systems against varroa will be developed by integrating resistant bee stock with other non-chemical means to manage varroa.