Project Number: 6036-22000-025-03
Start Date: Sep 30, 2014
End Date: Sep 29, 2019
PCR approaches will be taken to identify and isolate the transformer and doublesex sex determination genes from the Bactrocera species of interest using homologous sequences from B. dorsalis for primer design. Female-specific introns from the sex determination genes will then be incoporated into the Tet-transactivator (TTA) driver cassette. The hid and reaper pro-apoptotic cell death genes will be similarly isolated from the Bactrocera species, and their coding sequences will be linked in-frame to the Tet-response element (TRE) to create lethal effector cassettes. Both the driver and effector cassettes will be incorporated into piggyBac vectors and transformed into the host Bactrocera species. Progeny from appropriate matings between driver and lethal effector transformant lines will then be tested on Tet-containing and Tet-free diet to evaluate female-specific lethality in order to produce male-only lines for sterile male releases.