Location: Foreign Animal Disease Research
Project Number: 1940-32000-057-41
Start Date: Jun 01, 2012
End Date: May 31, 2014
1. Bovine immune cells will be cultured with live or inactivated viruses. FMDV-specific antibody production will be quantified and characterized from the culture supernatants and FMDV-specific antibody secreting cells will also be determined. Cell cultures will be sorted for specific surface cell markers to determine FMDV-specific antibodies. 2. The levels of interferon will be measured to determine antibody concentrations. Specific antibody detection will be performed to quantify the amount of stimulated cells and measure the magnitue of response multiple bond connection strenght. Activation markers will be detected through flow cytometery and to determine activation methodologies. 3. A recombinant antigen comprising 40 tandem copies (a multi-peptide construct) of the FMDV viral protein (VP)1 site A will be assessed as thymus (T)-cell independent antibody inducer to study on the structural features of FMDV T-cell independent antigens. Specifically, we will produce the recombinant antigen and will study the cellular requirement to induce the antibody response against it. If positive results are obtained, the immunogenicity of the recombinant antigen will be assessed in experimental model (mice) and natural host (cattle).