Location: Bee Research
Project Number: 1245-21000-277-04
Start Date: Jun 01, 2011
End Date: Jan 31, 2014
1) Continued genomic sequencing to 20x coverage with an ‘optimal’ mix of straight and end-pair 454 reads, followed by genome assembly, coordinated by USDA-ARS Beltsville colleagues, and colleagues at the University of Maryland for informatics help. 2) Transcriptome surveys using 454 pyrosequencing, focused on: a) nymphal development, b) host finding (tarsal library), c) immune responses (gut with and without virus infection), and d) gut microbes. 3) SNP and protein polymorphism discovery using the ABI SOLiD platform on the mite transcriptome. Mites will be gathered by (Midwestern US), (South), mid-Atlantic and Pacific west) as well as ‘outgroups’ from (Australia) and (France). These data will be aligned with homologous sequence data from the genome reads (Maryland mite), and from the 454 transcriptome reads to give an abundance of SNPs. 4) Development and testing of a canonical gene set. All in consortium involved, with help from (NCBI). 5) Integration and posting of emerging data via Beebase, NCBI, and other public databases with assistance from Purdue University, as a bridge to tick genomics and VectorBase).