Location: Horticultural Crops Disease and Pest Management Research Unit
Project Number: 2072-22000-044-056-A
Project Type: Cooperative Agreement
Start Date: Sep 1, 2025
End Date: Aug 31, 2027
Objective:
Arthropod pests cause over $120 billion in agricultural losses annually in the U.S., with invasive species posing an even greater threat due to the absence of natural predators. This project focuses on invasive insect pests, including the spotted lanternfly and box tree moth, aiming to develop an innovative, biologically based control method using advanced molecular technologies.
Approach:
1. Molecular cloning and sequencing of target genes: Target genes from selected insect pests will be cloned and sequenced. Candidate gene sequences will be validated through 3D GPCR structural modeling. Gene amplification will be performed using cDNA synthesized from total RNA, following standard molecular biology protocols.
2. In vitro expression and functional characterization of receptor genes: Receptor genes will be expressed in vitro and functionally characterized. The cooperator will provide expertise on species-specific biology and behavioral ecology of the target pests in field conditions.
3. Screening of bioactive molecules and insect bioassays: A range of biological molecules will be screened using receptor interference method. Bioassays with the cooperator will be conducted on target insects to evaluate the physiological impact and effects on survival.