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ARS Home » Pacific West Area » Parlier, California » San Joaquin Valley Agricultural Sciences Center » Crop Diseases, Pests and Genetics Research » Research » Research Project #443374

Research Project: Study of the Putative Vectors Associated with Citrus Yellow Vein Clearing Virus (CYVCV) discovered in Tulare County, California

Location: Crop Diseases, Pests and Genetics Research

Project Number: 2034-22000-015-002-I
Project Type: Interagency Reimbursable Agreement

Start Date: Oct 1, 2022
End Date: Aug 31, 2024

Determine the principal vector(s) of CYVCV in the Tulare outbreak area and relevant host reservoirs and evaluate vector population dispersal.

Insects will be collected by aspiration and established on plants in the insectary. Virus (CYVCV)-infected blind buds will be graft inoculated in citrus plants and/or mechanically inoculated in beans in a USDA-approved greenhouse facility. Virus detection will be by RT-PCR. Insect vectors will be transferred by camel-hair brush. Virus symptoms will be documented by pictures and titer measured by RT-qPCR. Insect vectors with be marked by SmartWater liquid fluorescent solution and released in the field for mark/recapture experiments. Marked insects will be deteched by UV light with stereo microscope to determine local spatial movement and dispersal of vectors.