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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Research Project #440746

Research Project: Genetic and Genomic Characterization of Host - Pathogen Interactions in Common Bean

Location: Grain Legume Genetics Physiology Research

Project Number: 2090-21220-003-001-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Aug 1, 2021
End Date: Jul 31, 2026

To facilitate disease control by identifying candidate genes underlying host resistance to fungal and viral pathogens in common bean.

1) Generate different common bean populations with inbred lines segregating for resistances to bean rust, Sclerotinia white mold, Bean common mosaic virus, and Beet curly top virus. 2) Phenotype the segregating individuals for reactions to the different pathogens using greenhouse and field screening protocols. 3) Genotype the individuals using single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) obtained by Next-Generation sequencing. 4) Use association and linkage mapping to identify genic regions and candidate genes. 5) Develop markers for marker-assisted selection of the genes. 6) Collect pathogen isolates and characterize them against a set of differential host genotypes and by genomic sequencing.