Location: Peanut and Small Grains Research Unit
Project Number: 3070-21220-009-009-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Apr 1, 2021
End Date: Mar 31, 2025
Objective:
The goal of this proposal is to advance an existing, partially-characterized peanut wild species introgression population and generate near-isogenic introgression lines (NIILs) in a cultivated background, providing phenotypic data on the breeding lines, and marker data for use in selection. These NIILs will serve as a living repository for germplasm of wild species in an easily-accessible form for peanut breeders, and will be propagated as homozygous lines to serve as a long-term resource for phenotypic analysis, breeding, and study of gene introgression.
Specific objectives are to:
1. Perform high-resolution SNP genotyping of the current backcross population.
2. Perform marker-assisted backcrossing to develop NIILs.
3. Phenotype NIILs for pest and disease resistance and seed quality traits, and identify QTLs
4. Increase seed of NIILs and release as a germplasm resource.
The ARS investigator's specific objective will be to phenotype the near-isogenic introgression lines for disease resistance.
Approach:
The near-isogenic introgression lines will be phenotyped in the field for early leafspot resistance at the Caddo Research Station in Fort Cobb, OK for three years. The near-isogenic introgression lines will be planted in plots consisting of two, 15'-long rows, using a randomized complete block design with three replications. Plots will be rated a minimum of two times during the field season by rating the percentage of leaves covered with leaf spot. Disease analyses will be conducted using mixed-model ANOVA in GLIMMIX (SAS, v. 4.0).
