Location: Plant, Soil and Nutrition Research
Project Number: 8062-21000-045-005-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Apr 19, 2021
End Date: Apr 18, 2023
Objective:
Crop improvement can be greatly facilitated by having the whole genome sequence of the crop. An international effort is underway in this regard to sequence multiple individuals of hexaploid oat, Avena sativa. The objective of this agreement is to sequence an important progenitor line of at, Clintland60, to contribution to that effort. Collaborators will use whole genome sequence on single-seed derived plants of the hexaploid Avena sativa, Clintland60, from seed held by the National Small Grains Collection. This sequence will become part of a broader effort to evaluate the complex genomic organization of cultivated milling oat in relation to that of it’s wild relatives, and to discover genomic variation that can be developed into molecular genetic tools for crop improvement. Results from this project will be integrated into a broader oat pangenome effort.
Approach:
One Avena accessions in the National Small Grains Collection (NSGC) has been identified as an important progenitor to a number of oat varieties used in the upper mid-west. This project will sequence that progenitor to ensure that haplotypes in its descendants are well represented in an oat pangenome project.
The ARS PIs will provide accesses to genomic sequence data on the accession, with assembly by the Oat Pangenome Project; and expertise on using the sequence for imputation of high density marker data in elite North American oat breeding lines. The University Cooperator will provide expertise on Avena cytogenetics and evolution and will perform the sequence comparison work. The ARS PI and University Cooperator will collaborate with each other and with oat researchers worldwide to annotate these genomes in the context of other oat genomes currently under analysis.
