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ARS Home » Plains Area » Fort Collins, Colorado » Center for Agricultural Resources Research » Plant Germplasm Preservation Research » Research » Research Project #431402

Research Project: RNA Integrity and Viability of Seeds and Preserved Germplasm

Location: Plant Germplasm Preservation Research

Project Number: 3012-21000-016-06-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2016
End Date: Aug 31, 2019

Objective:
The objectives of this research are to conduct experiments that test the correlation between RNA integrity and viability of stored seeds and other germplasm and to distinguish between dead versus dormant materials. The long-term objective is to develop efficient methods to preserve germplasm and to monitor viability during storage.

Approach:
Decline in viability of seeds or other germplasm forms from crop, native or invasive species will be correlated with fragment size of RNA extracted from a subset of the tested material. Mechanisms of RNA degradation will be explored by measuring the extent of non-specific oxidation. Specific patterns of RNA degradation will be explored using RNAseq studies.