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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Research Project #446308

Research Project: A Novel Genetic Strategy and Mechanism to Improve Tomato Fruit Yield

Location: Plant, Soil and Nutrition Research

Project Number: 8062-21000-050-031-I
Project Type: Interagency Reimbursable Agreement

Start Date: May 1, 2024
End Date: Apr 30, 2027

1) To examine the effects of SlOr family genes on fruit production via generation of tomato CRISPR/Cas9 knockout mutants; 2) To elucidate the mechanisms underlying the Or-affected fruit set; and 3) To investigate the association of SlOr family gene expression with fruit set in tomato germplasm.

Transgenic lines will be generated via A. tumefaciens-mediated transformation. Both conventional molecular and biochemical approaches will be employed for functional studies to understand how the Or gene regulates fruit set.