Project Number: 2034-22000-014-016-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jul 1, 2022
End Date: Jun 30, 2024
Identify and sequence Central Valley grapevine Trichoderma isolates that exhibit effective biocontrol of Pierce's disease of grapevine.
An already available unique collection of Central Valley Trichoderma isolates will be screened to identify those that enhance resistance to Pierce's disease. This will consist of greenhouse plant-pathogen assays whereby plants are first inoculated with Trichoderma isolates via stem or root inoculations, and then infected with Xylella fastidiosa, the causal agent of Pierce's disease, via the pin-prick method. Following two-, four- and six-months, disease measurements will be made and Xylella fastidiosa titers assessed via PCR in infected plants, and comparisons will be made with healthy controls. Next-generation sequencing and microscopy approaches also will be employed to genetically and morphologically characterize of Trichoderma biocontrol isolates.