Location: Corn Insects and Crop Genetics Research
Project Number: 5030-21220-006-056-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Jan 1, 2022
End Date: Dec 31, 2022
Objective:
Year three of the project will continue development and release of soybean cultivars and germplasm with resistance to Pythium, Fusarium graminearum, and/or brown stem rot pathogen Phialphora gregata, as well as to identify and characterize the candidate genes for development of ‘perfect’ molecular markers for rapid and precise incorporation of the targeted resistance into US germplasm.
Approach:
1. Cooperators will continue to develop new and improved soybean varieties and germplasm with enhanced resistance traits.
2. Cooperators and ARS will use mapping, whole genome expression analyses to identify the specific gene(s) contributing to resistance and determine how resistance is regulated.
3. Cooperators will use VIGS and CRIPSR/Cas9 technologies to confirm candidate genes.
4. ARS will use VIGS coupled with RNA-seq to confirm candidate genes and the molecular networks they control.
