Location: Plant Genetics Research
Project Number: 5070-21000-040-044-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Oct 1, 2021
End Date: Feb 28, 2023
Objective:
Develop and release experimental lines and varieties that are higher in protein concentration and higher in yield than current varieties.
Approach:
Soybean with high protein sources will be characterized for the presence of the major protein quantitative trait loci (QTL) on chromosomes 15 and 20. Protein composition and changes in gene expression will be characterized in selected experimental soybean lines. Using RNAseq experiments and 1-D and 2-D gel electrophoresis, followed by protein identification by mass spectrometry. Additionally, the concentration of sulfur-rich proteins (leginsulin, lunasin, Bowman-Birk protease inhibitor) in these experimental soybean lines will also be evaluated. Breeding populations derived from exotic germplasm with higher protein concentration will be crossed with existing highest yielding experimental lines.and analyzed for protein and yield.
