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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #441532

Research Project: Increasing Genetic Diversity, Yield, and Protein of US Commercial Soybean Germplasm

Location: Plant Genetics Research

Project Number: 5070-21000-040-044-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2021
End Date: Aug 31, 2022

Objective:
Develop and release experimental lines and varieties that are higher in protein concentration and higher in yield than current varieties.

Approach:
Soybean with high protein sources will be characterized for the presence of the major protein quantitative trait loci (QTL) on chromosomes 15 and 20. Protein composition and changes in gene expression will be characterized in selected experimental soybean lines. Using RNAseq experiments and 1-D and 2-D gel electrophoresis, followed by protein identification by mass spectrometry. Additionally, the concentration of sulfur-rich proteins (leginsulin, lunasin, Bowman-Birk protease inhibitor) in these experimental soybean lines will also be evaluated. Breeding populations derived from exotic germplasm with higher protein concentration will be crossed with existing highest yielding experimental lines.and analyzed for protein and yield.