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ARS Home » Plains Area » Lincoln, Nebraska » Wheat, Sorghum and Forage Research » Research » Research Project #441110

Research Project: Enriching and Understanding the Wheat Genome by Inducing Secondary Homoeologous Recombination

Location: Wheat, Sorghum and Forage Research

Project Number: 3042-21000-033-028-A
Project Type: Cooperative Agreement

Start Date: Aug 15, 2021
End Date: Mar 14, 2022

Objective:
1) Enhance B-S and B-E recombination toward proximal chromosomal regions and reduce alien segments in the favorable recombinants; 2) Construct composite bin maps of wheat B-genome chromosomes at a relatively high resolution; 3) Remove the ph1b mutant allele from the B-S and B-E recombinants and develop homozygous recombinant lines for germplasm development and genome studies; and 4) Evaluate the B-S and B-E recombinant lines for the traits of interest and develop breeding-ready germplasm in the adapted wheat backgrounds.

Approach:
We will continue making efforts toward the research goals through the following approaches: 1) Generate and recover new recombinants involving homoeologous B-S and B-E chromosome pairs in all groups using chromosome-specific DNA markers and GISH/FISH; 2) Induce secondary and tertiary homoeologous recombination to develop new recombinants toward the proximal chromosomal regions and involving smaller alien chromosomal segments; 3) Reduce the alien chromosomal segments containing the genes targeted in this project and physically map the genes of interest; 4) Develop user-friendly DNA markers diagnostic for the genes of interest and perform marker-assisted introgression of the wild species-derived genes into adapted wheat genotypes for breeding-ready germplasm development; 5) Genotype the recombinants using high-throughput SNP arrays and construct physical maps of wheat B-genome chromosomes; 6) Develop homozygous recombinant lines for trait evaluation and germplasm development.