Location: Poultry Microbiological Safety and Processing Research Unit
Project Number: 6040-32000-079-003-N
Project Type: Non-Funded Cooperative Agreement
Start Date: Oct 1, 2021
End Date: Dec 31, 2022
Objective:
The threat of Antimicrobial Resistance (AMR) is a major food safety and human health concern. Despite that agriculture is assumed to be the source of much of the development of AMR, very little data is available to determine this. There for research into the U.S. swine industry must be done to collect the data on AMR, pathogens and commensal bacteria, This project will develop a comprehensive strategy for collecting this data across an industrial swine production system.
Approach:
ARS scientists will aid the cooperator (Pipestone) researchers in developing this antibiotic use tracking and AMR monitoring system. Escherichia coli, Salmonella enterica, Campylobacter, and Enterococcus will be monitored and isolated for AMR analysis by ARS scientists. ARS scientist will analyze all resulting data from the study as well as perform whole genome sequence analysis on select isolates to collect data on the genetics of AMR. Additionally, Metagenome analysis of samples from production facilities will be analyzed to determine the organism in the swine industry effecting AMR. Reservoirs and donors of AMR genes will be identified by this analysis. These data and analyses will correlated with antimicrobial usage in the animals and help determine what effect use has on AMR development in swine.
