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ARS Home » Pacific West Area » Tucson, Arizona » Carl Hayden Bee Research Center » Research » Research Project #440868

Research Project: Pollen Identification and Analysis

Location: Carl Hayden Bee Research Center

Project Number: 2022-21000-022-051-I
Project Type: Interagency Reimbursable Agreement

Start Date: Jul 25, 2021
End Date: Sep 30, 2022

The goals of the project are to: 1) Identify the plant source of pollen samples collected by the research team at Oregon State University using meta-barcoding. 2) Quantify the concentration of omega 3 and 6 fatty acids in the pollen samples. 3) Provide the information to our collaborator at Oregon State University and to NRCS PLANTS database for inclusion in the new Pollinators section.

For Objectives 1and 2, standard, widely accepted protocols will be used for conducting meta-barcoding for plant identification, and analysis of omega 3 and 6 levels using fatty acid methyl ester (FAME) analysis. FAME compounds will be quantified by comparison of characteristic mass fragments with known amounts of authentic standards.