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ARS Home » Plains Area » Lubbock, Texas » Cropping Systems Research Laboratory » Livestock Issues Research » Research » Research Project #440339

Research Project: Evaluation of Oxidative Stress Markers in Beef Cattle Following a LPS Challenge

Location: Livestock Issues Research

Project Number: 3096-32000-008-002-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Feb 1, 2021
End Date: Jul 31, 2021

Determine the effect of MetaSmart, an ester analogue of methionine, fed during the receiving period, on oxidative stress markers in beef cattle exposed to a lipopolysaccharide (LPS) challenge.

Following a 35-day supplementation period, cattle will be fitted with indwelling jugular catheters and rectal temperature recording devices that will measure body temperature continuously at 5-min intervals. Cattle will then be placed in individual bleeding stalls. On day 42, all calves will be challenged intravenously with lipopolysaccharide (LPS; 0.25 µg/kg body weight; dose may vary due to condition of the cattle). Four blood samples will be collected at -2, 0, 2, 4, 6, 8, 10, 12, 18, 24, 36 and 48 h relative to the LPS challenge at time 0. One whole blood sample will be utilized for measuring hematology parameters, the second blood sample will be processed to harvest serum for analyzing cortisol and acute phase proteins the third blood sample will be processed to harvest plasma for a blood chemistry analysis, and indicators of oxidative stress. Oxidative stress marker analysis will include Reactive Oxygen Species, TBARS and FRAP.