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ARS Home » Southeast Area » Griffin, Georgia » Plant Genetic Resources Conservation Unit » Research » Research Project #440245

Research Project: Detecting ToBRFV in the USDA Capsicum Germplasm Collection - II

Location: Plant Genetic Resources Conservation Unit

Project Number: 6046-21000-012-023-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: May 1, 2021
End Date: Apr 30, 2022

Objective:
To index those Capsicum germplasm accessions that previously tested ‘very weakly positive’ via a general Tobamovrus indexing scheme, for the presence of ToBRFV.

Approach:
We propose to sample distribution seed inventories of Capsicum species in the S9 Capsicum spp. germplasm collection that previously tested ‘very weakly positive’ for the presence of Tobamoviruses. In this previous indexing, these samples failed to produce sufficient PCR product for sequencing and specific virus identification. An alternative assay specific for ToBRFV will be utilized. One hundred (100) seed of each Capsicum accession currently that previously tested ‘very weakly positive’ for the presence of Tobamoviruses will be packaged and labeled in Griffin and sent to AgDia testing. Samples testing positive for ToBRFV will be flagged in the GRIN database as ‘infected’ and the inventory no longer distributed or otherwise made available. Infected seed inventories will be destroyed. Inventories testing negative will be designated as such in the database.