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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #440198

Research Project: Downy Mildew Detection, Epidemiology, and Biopesticide Evaluation

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-019-024-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Apr 1, 2021
End Date: Sep 30, 2022

Objective:
Examine Peronospora effusa oospore production and seed transmission, compare the effectiveness two types of spore traps for airborne detection of P. effusa and Bremia lactucae, and complete biopesticide testing for control of spinach downy mildew in microplot experiments.

Approach:
Seed transmission of downy mildew disease on spinach will be assessed in glass isolators to limit external contamination from airborne spores. Spinach seed with oospores will be planted in the isolators and evaluated for disease six weeks after emergence. We will deploy the two spore trap types at a field site in Salinas, California, to collect airborne spores of B. lactucae and P. effusa. and conduct quantitative PCR analysis to determine quantities of pathogen DNA detected. Pathogen quantities will be compared between trap types over time by linear regression. The biopesticide testing will be conducted outdoors in microplots. Spinach will be planted at commercial standard density and evaluated for disease incidence in biopesticide treated and non treated plots.