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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #439670

Research Project: Optimization of Techniques for Detection and Quantification of Soilborne Pathogens using Molecular Techniques

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-016-054-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Feb 1, 2021
End Date: Jan 31, 2022

The specific objectives of this proposal encompass research to optimize molecular soil quantification assays for V. dahliae, M. phaseolina and F. oxysporum f. sp. fragariae by optimizing strategies for collection of field samples to ensure quantification assays are accurate, and evaluate how long DNA in nonviable propagules remains intact.

Molecular quantification assays have been developed for the indicated soilborne pathogens of strawberry that enable for pathogen quantification in field soils. To effectively use these techniques however additional research needs to be conducted to optimize sampling techniques to ensure results accurately reflect pathogen distribution in the field. High density, hierarchical sampling for infected fields will be done and the results examined to evaluate the most accurate sampling approach that can be used. Fields will also be examined after fumigation to evaluate the length of time it takes for DNA in nonviable survival structures to degrade and not be detected by the molecular assays.