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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #439303

Research Project: Improved Methods to Measure Trypsin Inhibitor Levels and Activity in Soybean Meal

Location: Plant Genetics Research

Project Number: 5070-21000-040-40-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2020
End Date: Sep 30, 2021

Objective:
The objective of this project is to develop a simple, reliable and inexpensive procedure that can be routinely utilized by soybean processors as a standard method to measure trypsin inhibitor levels.

Approach:
We propose to develop an easy, reliable, and rapid measurement of trypsin inhibitor activity/content in soybean meal using dye-tagged protein substrates, that will enhance the fluorescence of the dye when broken down into peptides. The aim is to develop a kit that can be utilized at the elevators and feed processing facilities to reliably and routinely measure trypsin inhibitor activity in the soybean meal.