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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Research » Research » Research Project #439065

Research Project: Fungicide Resistance, Hormesis, and Fungal Pathogens in Controlled Environment Crops

Location: Horticultural Crops Research

Project Number: 2072-22000-043-51-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2020
End Date: Oct 31, 2022

Objective:
1. Screen for diversity of fungal pathogen species and populations. 2. Examine incidence of fungicide resistance. 3. Characterize hormesis responses.

Approach:
1. Plant pathogens, such as Phytophthora, will be sampled from plants grown in controlled environments such as nurseries and greenhouses in 2020 and 2021. Pathogens will be cultured and identified to species. Populations will be characterized using DNA sequence and genetic markers. 2. Fungicide resistance will be examined in pathogen populations identified in 2021 and 2022 by growing cultures on fungicide amended media. Pathogen strains will be identified as resistant or sensitive to fungicides. 3. Pathogens will be examined for hormesis in 2021 and 2022. Pathogens will be grown on fungicide amended plates at very low fungicide doses to determine if there is a fungicide concentration that stimulates growth. Pathogens will be grown at a stimulatory dose and compared to nonamended controls. Messenger RNA will be extracted, sequenced and compared between treatments.