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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #438998

Research Project: 6.0179 Expanding Non-transgenic Genetic Sexing Systems to New Pests Tephritids

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-027-002-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2020
End Date: Sep 29, 2021

Develop novel genetic sexing systems in tephritid fruit flies to support enhanced sterile insect technique programs.

Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Veinna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to re-creating temperature sensitive lethal in Anastrepha or Bactrocera species.