Skip to main content
ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #438998

Research Project: 6.0179 Expanding Non-transgenic Genetic Sexing Systems to New Pests Tephritids

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-027-002-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2020
End Date: Sep 29, 2021

Objective:
Develop novel genetic sexing systems in tephritid fruit flies to support enhanced sterile insect technique programs.

Approach:
Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Veinna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to re-creating temperature sensitive lethal in Anastrepha or Bactrocera species.