Skip to main content
ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Pest Genetics and Molecular Biology Research Unit » Research » Research Project #438661

Research Project: Expanding Non-transgenic Genetic Sexing Systems to New Pests Tephritids

Location: Tropical Pest Genetics and Molecular Biology Research Unit

Project Number: 2040-22430-028-001-I
Project Type: Interagency Reimbursable Agreement

Start Date: Aug 1, 2020
End Date: Jan 27, 2022

Develop novel genetic sexing systems in tephritid fruit flies to support enhanced sterile insect technique programs.

Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Vienna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to recreating temperature sensitive lethal in Anastrepha or Bactrocera species.