Skip to main content
ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Pest Genetics and Molecular Biology Research Unit » Research » Research Project #438661

Research Project: Expanding Non-transgenic Genetic Sexing Systems to New Pests Tephritids

Location: Tropical Pest Genetics and Molecular Biology Research Unit

Project Number: 2040-22430-028-001-I
Project Type: Interagency Reimbursable Agreement

Start Date: Aug 1, 2020
End Date: Jan 27, 2022

Objective:
Develop novel genetic sexing systems in tephritid fruit flies to support enhanced sterile insect technique programs.

Approach:
Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Vienna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to recreating temperature sensitive lethal in Anastrepha or Bactrocera species.