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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #438661

Research Project: Expanding Non-transgenic Genetic Sexing Systems to New Pests Tephritids

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-026-64-I
Project Type: Interagency Reimbursable Agreement

Start Date: Aug 1, 2020
End Date: Jan 27, 2022

Objective:
Develop novel genetic sexing systems in tephritid fruit flies to support enhanced sterile insect technique programs.

Approach:
Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Vienna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to recreating temperature sensitive lethal in Anastrepha or Bactrocera species.