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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #438659

Research Project: 3.0351.03 Resolving Species Complexes of Bactrocera Fruit Flies FY20

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-026-63-I
Project Type: Interagency Reimbursable Agreement

Start Date: Aug 1, 2020
End Date: Jan 27, 2022

Objective:
Develop diagnostic tools for discrimination of species within species complexes in the genus Bactrocera, and transferring these tools to APHIS for implementation in their pest detection programs.

Approach:
Using specimens identified by expert taxonomists (through University of Hawaii Manoa collaboration), perform sequencing of barcode region (COI) as well as multigene phylogenomics on several species complexes within the genus Bactrocera (B. frauenfeldi/albistrigata complex, B. tau complex, and B. dorsalis complex). From these datasets, we hope to resolve/refine the complexes. In addition, we will then develop diagnostic tools/assays for discriminating species within these complexes, including developing both wet-lab and bioinformatic tools for rapid implementation and analysis of these assays.