Location: Diet, Genomics and Immunology Laboratory
Project Number: 8040-51530-057-016-I
Project Type: Interagency Reimbursable Agreement
Start Date: Aug 13, 2020
End Date: Aug 12, 2021
Objective:
To elucidate the mechanisms underlaying cancer protective effects of the cruciferous vegetable-derived compound Indole-3-carbinol (I3C).
Approach:
Experiment 1: Identify and validate specific T-cell populations (ex. Cytotoxic T-cell, T-reg etc.) affected by dietary I3C. The ultimate goal is to examine how I3C modulates these T-cell subsets in C rodentium-infected mouse model. We hypothesized that I3C could protect against C rodentium-induced inflammation. Therefore, we will evaluate the effect of I3C on the relevant peripheral immune-regulating mechanisms in C rodentium-infected mice.
Experiment 2: Elucidate temporal effects of dietary I3C on the responsive T-cell subsets. Six-week-old C57BL/J6 mice will be fed the control or experimental diet for 14 days, and then infected with C rodentium. Feces will be collected from all groups to determine the level of C rodentium fecal excretion. Blood T-cells will be determined using flow cytometry to elucidate changes in specific immune cell population, as well as temporal effects on the immune system. Furthermore, serum and/or colonic cytokines level can be determined to corroborate with flow cytometry results.
