Location: Foreign Animal Disease Research
Project Number: 8064-32000-063-017-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jun 1, 2020
End Date: May 31, 2022
This collaborative agreement seeks to design and synthesize synthetic DNA encoding for African swine fever virus to be used for creating recombinant ASFV.
African Swine Fever (ASF) is a large DNA virus of approximately 180kb. The ASFV-G genome will be synthesized from de-novo synthesis, using patented methods from CodexDNA, containing a fluorescent marker. Mutants will also be made changing particular genes from genotype II to a different genotype. The DNA will then be used along with techniques involving ARS-PIADC's propriety helper virus to create infectious ASFV virus from de-novo synthesis.