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ARS Home » Northeast Area » Orient Point, New York » Plum Island Animal Disease Center » Foreign Animal Disease Research » Research » Research Project #438304

Research Project: Development of Synthetic African Swine Fever Viruses to Determine the Role of Structural Viral Proteins

Location: Foreign Animal Disease Research

Project Number: 8064-32000-060-33-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jun 1, 2020
End Date: May 31, 2021

Objective:
This collaborative agreement seeks to design and synthesize synthetic DNA encoding for African swine fever virus to be used for creating recombinant ASFV.

Approach:
African Swine Fever (ASF) is a large DNA virus of approximately 180kb. The ASFV-G genome will be synthesized from de-novo synthesis, using patented methods from CodexDNA, containing a fluorescent marker. Mutants will also be made changing particular genes from genotype II to a different genotype. The DNA will then be used along with techniques involving ARS-PIADC's propriety helper virus to create infectious ASFV virus from de-novo synthesis.