Location: Plant Stress and Germplasm Development Research
Project Number: 3096-21000-022-031-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Jul 1, 2020
End Date: May 31, 2022
Objective:
Assess the level of FOV4 tolerant/resistant cotton lines with improved tolerance/resistance to FOV4 in far-west Texas under infested fields and greenhouse conditions.
Approach:
Selected progeny, breeding lines and/or germplasm with diverse levels of stress or disease resistance/tolerance to Fusarium wilt with diverse plant-architecture or root-morphology will be evaluated in infested FOV4 fields and artificial inoculation under greenhouse conditions. The breeding material or cotton entries were selected/developed/advanced through traditional breeding procedures. These improved lines targeted introgression of FOV4 resistance/tolerance genes from diverse genetic backgrounds. These developed cotton entries and additional entries of interest were identified from ongoing screening and selection efforts from a cooperative project by the USDA-ARS and University cooperators. Responses from greenhouse and field test-evaluations will be examined using ANOVA or nonparametric analyses as appropriate. Field evaluations will use RCBD or incomplete block designs with 3–4 replications using five plants as subsamples per replication. In addition, this project will help to support the discovery of molecular markers or biomarkers for assisting breeding. Selected germplasm with diverse levels of resistance-response to FOV4 will be subjected to the next-generation sequencing (NGS) technology for gene expression analyses and SNP discovery.
